SAGE Journals

Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation

Posted on 2020-08-01 - 14:06

The cerebral cortex has a number of conserved morphological and functional characteristics across brain regions and species. Among them, the laminar differences in microvascular density and mitochondrial cytochrome c oxidase staining suggest potential laminar variability in the baseline O2 metabolism and/or laminar variability in both O2 demand and hemodynamic response. Here, we investigate the laminar profile of stimulus-induced intravascular partial pressure of O2 (pO2) transients to stimulus-induced neuronal activation in fully awake mice using two-photon phosphorescence lifetime microscopy. Our results demonstrate that stimulus-induced changes in intravascular pO2 are conserved across cortical layers I–IV, suggesting a tightly controlled neurovascular response to provide adequate O2 supply across cortical depth. In addition, we observed a larger change in venular O2 saturation (ΔsO2) compared to arterioles, a gradual increase in venular ΔsO2 response towards the cortical surface, and absence of the intravascular “initial dip” previously reported under anesthesia. This study paves the way for quantification of layer-specific cerebral O2 metabolic responses, facilitating investigation of brain energetics in health and disease and informed interpretation of laminar blood oxygen level dependent functional magnetic resonance imaging signals.


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Journal of Cerebral Blood Flow & Metabolism


İkbal Şencan
Tatiana Esipova
Kıvılcım Kılıç
Baoqiang Li
Michèle Desjardins
Mohammad A Yaseen
Hui Wang
Jason E Porter
Sreekanth Kura
Buyin Fu
Timothy W Secomb
David A Boas
Sergei A Vinogradov
Anna Devor
Sava Sakadžić
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